Suvà Lab

Overview

The Suvà laboratory develops and applies multi-dimensional spatial genomic, single-cell multi-omic and advanced computational analyses to dissect the biology of adult and pediatric gliomas. We study clinical samples at single-cell resolution and establish genetically and epigenetically faithful cellular models directly from patient tumors. We model how brain cancer cells exploit their plasticity to establish phenotypically distinct populations of cells, with a focus on malignant cell states that we defined. We seek to redefine tumor cell lineages, stem cell programs and immune cells subsets across all subtypes of gliomas, and to leverage the information for renewed therapeutics. The laboratory additionally leverages single-cell and spatial genomics to characterize the cellular and molecular response of brain tumors to experimental therapies.

Research Team

Ester Calvo Fernandez, PhD
Yilin Fan, PhD
Demi Gerovasilis, BS
Nick Gonzalez-Castro, MD, PhD
Alexander Jucht, MD-PhD graduate student
Sophia Kovatsis, BS
John Lee, PhD
Vamsi Mangena, PhD
Chris Mount, MD, PhD
Mario L. Suvà, MD, PhD
Min Yang, PhD
Jun Zhong, BS

Publications

1. Spitzer AS†, Johnson KC†, Nomura M†, Garofano L†, Nehar-belaid D, Galili Darnell N, Greenwald AC, Bussema L, Oh YT, Varn FS, D’Angelo F, Gritsch S, Anderson KJ, Migliozzi S, Gonzalez Castro LN, …, Yung WKA, Lasorella A, Iavarone A, Verhaak RGW, Tirosh I, Suvà ML. Deciphering the Longitudinal Trajectories of Glioblastoma Ecosystems by Integrative Single-Cell Genomics. Nature Genetics. 2025 May 9.
2. Nomura M†, Spitzer AS†, Johnson KC†, Garofano L†, Nehar-belaid D, Galili Darnell N, Greenwald AC, Bussema L, Oh YT, Varn FS, D’Angelo F, Gritsch S, Anderson KJ, Migliozzi S, Gonzalez Castro LN, …, Yung WKA, Lasorella A, Iavarone A, Verhaak RGW, Suvà ML*, Tirosh I*. The Multi-Layered Transcriptional Architecture of Glioblastoma Ecosystems. Nature Genetics. 2025 May 9.
3. Mangena V†, Chanoch-Myers R†, Sartore R, Paulsen B, Gritsch S, Weisman H, Hara T, Breakefield XO, Breyne K, Regev A, Chung K, Arlotta P, Tirosh I, Suvà ML. Glioblastoma-cortical organoids recapitulate cell state heterogeneity and intercellular transfer. Cancer Discovery. 2024 Oct 7.
4. Greenwald AC†, Galili-Darnell N†, Hoefflin R†, Simkin D, Mount CW, Gonzalez-Castro LN, Harnik Y, Dumont S, Hirsch D, Nomura M, Talpir T, Kedmi M, Goliand I, Medici G, Laffy J, Li B, Mangena V, Keren-Shaul H, Weller M, Addadi Y, Neidert MC, Suvà ML*, Tirosh I*. Integrative spatial analysis reveals a multi-layered organization of glioblastoma. Cell. 2024 April 22.
5. Mathewson ND†, Ashenberg O†, Tirosh I†, Gritsch S†, Perez EM†, Marx S†, Jerby-Arnon L, Chanoch-Myers R, Hara T,…Louis DN, Ligon KL, Marson A, Chiocca EA, Reardon DA, Regev A*, Suvà ML*, Wucherpfennig KW*. Inhibitory CD161 Receptor Identified in Glioma-infiltrating T cells by Single Cell Analysis. Cell. 2021 Mar 4;184(5):1281-1298.
6. Neftel C†, Laffy J†, Filbin MG†, Hara T†, Shore ME, Rahme GJ, Richman AR, Silverbush D, Shaw ML, Hebert CM, Dewitt J, Gritsch S, Perez L, Gonzalez Castro LN,…Louis DN, Regev A, Bernstein BE, Tirosh I*, Suvà ML*. An integrative model of cellular states, plasticity and genetics for glioblastoma. Cell. 2019 Aug 8;178(4).

*Co-senior authorship
†Co-first authorship